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Arcella is an
amoeba that lives in a shell made of
chitin, the
same material that makes up the
exoskeleton
of insects. It eats bacteria
and small
algae. You can obtain a culture of harmless bacteria, such as
Enterobacter aerogenes (a normal inhabitant of the human gut) to
serve as food. Divide the Arcella population into several batches,
and add bacteria to half of them. After several days, use a microscope
to observe a sample drop from each batch that is placed on a microscope
slide and a cover slip added. Which Arcella are growing best?
Obtain cultures of Didinium, Paramecium caudatum (which
lacks
green algal
symbionts), and Paramecium bursaria (a species
that contains many
green algal
cells). Split the Didinium culture
into several batches. Add Paramecium caudatum to half the batches,
and Paramecium bursaria to the other half. After a few days, observe
samples from each batch with the microscope. In which batches is Didinium
most abundant and healthy? Which Paramecium is more tasty to the
Didinium (which is the antelope and which is the spiny anteater
of the
protoplankton world)? Does having
symbiotic algae make a difference?
If your microscope has a videocamera attachment, you can capture Didinium's
taste tests.
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The
amoeba Arcella has a rigid, protective shell with a hole through
which the
amoeba extends part of its cell to gather food. |
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The predatory ciliate Didinium consumes other ciliate
protozoa.
It's a "lion" in the world of
protoplankton. |
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